HLAG antibody

Catalog No. ABIN238406

Product Details anti-HLAG Antibody

Target: HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLAG antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Specificity: The antibody G233 recognizes several isoforms of HLA-G expressed in all populations of extravillous trophoblast (cell columns, interstitial trophoblast, endovascular trophoblast, placental bed giant cells). HLA-G belongs to the nonclassical MHC Class I molecules (MHC Class Ib). The antibody G233 has been found not to cross-react with any other MHC Class I antigens (HLA-A, -B, -C, -E, -F).

Cross-Reactivity (Details): Species reactivity (tested):Human

Characteristics: Synonyms: HLA-6.0, HLAG, MHC class I antigen G, HLA class I histocompatibility antigen

Purification: Protein A affinity chromatography

Purity: > 95 % by SDS-PAGE

Immunogen: Murine L cells transfected with both human beta2-microglobulin and HLA-G

Clone: G233

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry. Immunoprecipitation. ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C. Avoid repeated freezing andthawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for HLAG

Target: HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))

Alternative Name: HLA Class I alpha G / HLA-G (HLAG Products)

Synonyms: MHC-G antibody, B-F antibody, B-F-S04 antibody, B-F-S05 antibody, B-F-S06 antibody, B-F-S07 antibody, B-FI antibody, B-FIV antibody, BF2 antibody, BFa2 antibody, BFw-03 antibody, BFw-05 antibody, BFz-01 antibody, major histocompatibility complex, class I, G antibody, MHC BF1 class I antibody, HLA-G antibody, BF1 antibody

Background: HLA G belongs to the HLA class I heavy chain paralogues. It is a heterodimer consisting of a heavy chain (with a1, a2, a3 domains) and a light chain (beta 2 microglobulin). Alternate splicing provides both soluble and membrane bound forms of HLA G, with two soluble isoforms and four membrane bound isoforms. HLA G may be part of the fetal protection system and is expressed on fetal derived placental cells.Synonyms: HLA class I histocompatibility antigen, HLA-6.0, HLAG, MHC class I antigen G

Gene ID: 3135

UniProt: P17693

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints