for Flow Cytometry analysis of human blood cells using 10 m l reagent / 100 m l of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Buffer
PBS containing 15 mM sodium azide as preservative and 0.2 % (w/v) high-grade BSA (Protease free) as stabilizer
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C
Storage Comment
Store the antibody in the dark at 2-8 °C. Do Not Freeze! Avoid prolonged exposure to light. Shelf Life: one year from despatch.
CD10 (neutral endopeptidase - NEP, common acute lymphocytic leukemia antigen - CALLA, membrane metallo-endopeptidase - MME, enkefalinase) is a 100- kDa cell surface zinc metalloprotease cleaving peptide bonds on the N-terminus of hydrophobic amino acids and inactivating multiple physiologically active peptids. CD10 is expressed on various normal cell types, including lymphoid precursor cells, germinal center B lymhocytes, and some epithelial cells, and its expression level serves as a marker for diagnostics of many carcinomas. CD10 is also a differentiation antigen for early B-lymphoid progenitors in the B-cell differentiation pathway and has a key role in regulation of growth, differentiation and signal transduction of many cellular systems.Synonyms: Atriopeptidase, CALLA, Common acute lymphocytic leukemia antigen, EPN, Enkephalinase, MME, NEP, Neutral endopeptidase, Neutral endopeptidase 24.11