S100A8 antibody

Catalog No. ABIN238497

The Mouse Monoclonal anti-S100A8 antibody (Clone S13-67) (ABIN238497) specifically detects S100A8 in WB, FACS, IHC (p), IHC (fro) and EIA. The antibody is reactive with Human, Rat and Pig samples.

Quick Overview for S100A8 antibody (ABIN238497)

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Reactivity: Human, Rat, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This S100A8 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: S13-67

Product Details anti-S100A8 Antibody

Purification: Purified

Immunogen: Cultured human monocytes, the antigen is MRP8.

Isotype: IgG1

Application Details

Application Notes: Flow cytometry. ELISA. Dot blots. Immunohistochemistry on acetone-fixed frozen sections at 1-2 μg/mL (1: 200 - 1: 100) andparaffin sections at 4 μg/mL (1: 50), pretreatment is not necessary. Suggested positive control: Human tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.89 mg/mL

Buffer: PBS, pH 7.2 with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: Do Not Use Sodium Azide as preservative.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Target Details for S100A8

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Alternative Name: S100A8 / Calgranulin-A / MRP8

Background: Synonyms: CAGA, CFAG, Calprotectin L1L subunit, Cystic fibrosis antigen, Leukocyte L1 complex light chain, MRP-8, Migration inhibitory factor-related protein 8, S100 calcium-binding protein A8, S100-A8, Urinary stone protein band A

Gene ID: 9606

UniProt: P05109

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins