PCNA antibody

Catalog No. ABIN239835

The Mouse Monoclonal anti-PCNA antibody (Clone PC10) (ABIN239835) specifically detects PCNA in WB, IHC, FACS, IP and IHC (p). The antibody is reactive with Human and Mouse samples.

Quick Overview for PCNA antibody (ABIN239835)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This PCNA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: PC10

Product Details anti-PCNA Antibody

Specificity: Recombinant rat PCNA

Purification: Affinity purified

Immunogen: Recombinant human PCNA.

Type of Immunogen: Recombinant protein

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo, IHC, IHC-P (10 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Phosphate-buffered solution,  pH 7.2, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 4°C. Do not freeze.

Target Details for PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Alternative Name: PCNA / Cyclin

Background: Name/Gene ID: PCNA

Synonyms: PCNA, Cyclin

Gene ID: 5111

UniProt: P12004

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy