NOS1 antibody (AA 1422-1433)

Catalog No. ABIN2451595

Product Details anti-NOS1 Antibody

Target: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Binding Specificity: AA 1422-1433

Reactivity: Human, Mouse, Monkey, Dog, Rabbit, Bat, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human nNOS amino acids 1422-1433 (ESKKDTDEVFSS)1,2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Marmoset, Mouse, Dog, Bat, Elephant, Horse, Rabbit (100%) Rat, Chicken (92%) Xenopus, Zebrafish (83%).

Purification: Immunoaffinity purified

Immunogen: Human nNOS amino acids 1422-1433 (ESKKDTDEVFSS). Percent identity by BLAST analysis: Human, Gorilla, Marmoset, Mouse, Dog, Bat, Elephant, Horse, Rabbit (100%), Rat, Chicken (92%), Xenopus, Zebrafish (83%).

Isotype: IgG

Application Details

Application Notes: Approved: ICC, IHC, IHC-P (5 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: TBS,  pH 7.4, containing 50 % glycerol, 0.1 % BSA, and 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for NOS1

Target: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Alternative Name: NOS1 / nNOS (NOS1 Products)

Synonyms: NOS1 antibody, IHPS1 antibody, N-NOS antibody, NC-NOS antibody, NOS antibody, bNOS antibody, nNOS antibody, 2310005C01Rik antibody, NO antibody, NOS-I antibody, Nos-1 antibody, nos antibody, ihps1 antibody, nnos antibody, nos1 antibody, nos1-A antibody, nanos C2HC-type zinc finger 1 antibody, nitric oxide synthase 1 antibody, nitric oxide synthase 1, neuronal antibody, nitric oxide synthase 1 (neuronal) antibody, nitric oxide synthase 1 L homeolog antibody, NANOS1 antibody, NOS1 antibody, Nos1 antibody, nos1 antibody, nos1.L antibody

Background: Name/Gene ID: NOS1

Synonyms: NOS1, BNOS, Constitutive NOS, IHPS1, NNOS, NC-NOS, Nitric oxide synthase, brain, N-NOS, Neuronal NOS, NOS, NOS type I, Nos, type i

Gene ID: 4842

UniProt: P29475

Pathways: Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction