PHD1 antibody

Catalog No. ABIN2458111

Product Details anti-PHD1 Antibody

Target: PHD1 (EGLN2) (Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHD1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EGLN2.

Application Details

Application Notes: EGLN2 antibody can be used for detection of EGLN2 by ELISA at 1:62500. EGLN2 antibody can be used for detection of EGLN2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store EGLN2 antibody at -20 °C.

Target Details for PHD1

Target: PHD1 (EGLN2) (Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2))

Alternative Name: EGLN2 (EGLN2 Products)

Synonyms: egln2 antibody, phd2 antibody, PHD1 antibody, 0610011A13Rik antibody, C85656 antibody, Hif-p4h-1 antibody, Ier4 antibody, Phd1 antibody, SM-20 antibody, HIF-PH1 antibody, HPH-1 antibody, HPH-3 antibody, PHD-1 antibody, EIT6 antibody, HIFPH1 antibody, egl-9 family hypoxia-inducible factor 1 antibody, egl-9 family hypoxia inducible factor 2 antibody, Egl nine homolog 2 antibody, egl-9 family hypoxia-inducible factor 2 L homeolog antibody, egl-9 family hypoxia-inducible factor 2 antibody, egln1 antibody, EGLN2 antibody, egln2 antibody, egln2.L antibody, Egln2 antibody

Background: The hypoxia inducible factor (HIF) is a transcriptional complex which is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. EGLN2 encodes an enzyme responsible for this posttranslational modification. Alternative splicing of EGLN2 results in three transcript variants encoding different isoforms.

Molecular Weight: 16 kDa

Gene ID: 112398

UniProt: Q96KS0

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Cell RedoxHomeostasis