ATF3 antibody
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- Target See all ATF3 Antibodies
- ATF3 (Activating Transcription Factor 3 (ATF3))
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This ATF3 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA
- Purification
- Antibody is purified by peptide affinity chromatography method.
- Immunogen
- Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ATF3.
- Top Product
- Discover our top product ATF3 Primary Antibody
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- Application Notes
- ATF3 antibody can be used for detection of ATF3 by ELISA at 1:312500. ATF3 antibody can be used for detection of ATF3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
- Concentration
- 1 mg/mL
- Buffer
- Antibody is lyophilized in PBS buffer with 2 % sucrose.
- Handling Advice
- As with any antibody avoid repeat freeze-thaw cycles.
- Storage
- 4 °C/-20 °C
- Storage Comment
- For short periods of storage (days) store at 4 °C. For longer periods of storage, store ATF3 antibody at -20 °C.
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- Target
- ATF3 (Activating Transcription Factor 3 (ATF3))
- Alternative Name
- ATF3 (ATF3 Products)
- Synonyms
- zgc:55526 antibody, ATF3 antibody, LRG-21 antibody, LRF-1 antibody, LRFI antibody, atf-3 antibody, activating transcription factor 3 antibody, activating transcription factor 3 L homeolog antibody, atf3 antibody, ATF3 antibody, Atf3 antibody, atf3.L antibody
- Background
- This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. ATF3 represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters.Activating transcription factor 3 is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. Multiple transcript variants encoding two different isoforms have been found for this gene. The longer isoform represses rather than activates transcription from promoters with ATF binding elements. The shorter isoform (deltaZip2) lacks the leucine zipper protein-dimerization motif and does not bind to DNA, and it stimulates transcription presumably by sequestering inhibitory co-factors away from the promoter. It is possible that alternative splicing of the ATF3 gene may be physiologically important in the regulation of target genes.
- Molecular Weight
- 20 kDa
- Gene ID
- 467
- NCBI Accession
- NP_001665
- UniProt
- P18847
- Pathways
- Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Unfolded Protein Response
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