PIGO
Reactivity: Human
ELISA, WB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
PIGO antibody can be used for detection of PIGO by ELISA at 1:1562500. PIGO antibody can be used for detection of PIGO by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
Concentration
1 mg/mL
Buffer
Antibody is lyophilized in PBS buffer with 2 % sucrose.
Handling Advice
As with any antibody avoid repeat freeze-thaw cycles.
Storage
4 °C/-20 °C
Storage Comment
For short periods of storage (days) store at 4 °C. For longer periods of storage, store PIGO antibody at -20 °C.
Target
PIGO
(Phosphatidylinositol Glycan Anchor Biosynthesis, Class O (PIGO))
HPMRS2 antibody, RP11-182N22.4 antibody, mFLJ00350 antibody, phosphatidylinositol glycan anchor biosynthesis class O antibody, phosphatidylinositol glycan anchor biosynthesis, class O antibody, PIGO antibody, Pigo antibody
Background
PIGO is a protein that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid which contains three mannose molecules in its core backbone. The GPI-anchor is found on many blood cells and serves to anchor proteins to the cell surface. PIGO is involved in the transfer of ethanolaminephosphate (EtNP) to the third mannose in GPI.This gene encodes a protein that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid which contains three mannose molecules in its core backbone. The GPI-anchor is found on many blood cells and serves to anchor proteins to the cell surface. This protein is involved in the transfer of ethanolaminephosphate (EtNP) to the third mannose in GPI. At least two alternatively spliced transcripts encoding distinct isoforms have been found for this gene.