alpha KGDHC antibody

Catalog No. ABIN2460086

Product Details anti-alpha KGDHC Antibody

Target: alpha KGDHC (alphaKGDHC) (alpha Ketoglutarate Dehydrogenase (alphaKGDHC))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha KGDHC antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human OGDH.

Application Details

Application Notes: OGDH antibody can be used for detection of OGDH by ELISA at 1:12500. OGDH antibody can be used for detection of OGDH by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store OGDH antibody at -20 °C.

Target Details for alpha KGDHC

Target: alpha KGDHC (alphaKGDHC) (alpha Ketoglutarate Dehydrogenase (alphaKGDHC))

Alternative Name: OGDH (alphaKGDHC Products)

Synonyms: AKGDH antibody, E1k antibody, OGDC antibody, 2210403E04Rik antibody, 2210412K19Rik antibody, AA409584 antibody, d1401 antibody, mKIAA4192 antibody, oxoglutarate dehydrogenase antibody, oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) antibody, OGDH antibody, Ogdh antibody

Background: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).This gene encodes one subunit of the 2-oxoglutarate dehydrogenase complex. This complex catalyzes the overall conversion of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA and CO (2) during the Krebs cycle. The protein is located in the mitocondrial matrix and uses thiamine pyrophosphate as a cofactor. A congential deficiency in 2-oxoglutarate dehydrogenase activity is believed to lead to hypotonia, metabolic acidosis, and hyperlactatemia.

Molecular Weight: 47 kDa

Gene ID: 4967

NCBI Accession: NP_001003941

UniProt: Q96DD3