GFI1B
Reactivity: Human, Mouse, Rat
WB, IF (p), IHC (p)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
GFI1B antibody can be used for detection of GFI1B by ELISA at 1:312500. GFI1B antibody can be used for detection of GFI1B by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
Concentration
1 mg/mL
Buffer
Antibody is lyophilized in PBS buffer with 2 % sucrose.
Handling Advice
As with any antibody avoid repeat freeze-thaw cycles.
Storage
4 °C/-20 °C
Storage Comment
For short periods of storage (days) store at 4 °C. For longer periods of storage, store GFI1B antibody at -20 °C.
GFI1B acts in the late stage of erythroid differentiation as a transcriptional repressor. GATA-1 and NF-Y both contribute to erythroid-specific transcriptional activation of the Gfi-1B promoter. This zinc finger protein mediates erythroid expansion and has a role in normal erythropoiesis.