NKX2-8 antibody

Catalog No. ABIN2460297

Product Details anti-NKX2-8 Antibody

Target: NKX2-8 (NK2 Homeobox 8 (NKX2-8))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NKX2-8 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NKX2-8.

Application Details

Application Notes: NKX2-8 antibody can be used for detection of NKX2-8 by ELISA at 1:12500. NKX2-8 antibody can be used for detection of NKX2-8 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store NKX2-8 antibody at -20 °C.

Target Details for NKX2-8

Target: NKX2-8 (NK2 Homeobox 8 (NKX2-8))

Alternative Name: NKX2-8 (NKX2-8 Products)

Synonyms: NKX2.8 antibody, NKX2H antibody, Nkx2-9 antibody, Nkx-2.9 antibody, Nkx2-8 antibody, Nkx2.9 antibody, tinman antibody, NK2 homeobox 8 antibody, NK2 homeobox 9 antibody, NKX2-8 antibody, Nkx2-9 antibody, Nkx2-8 antibody

Background: A murine NKX2-8 was isolated from the Hepal-6 cell line and showed oligonucleotide binding competitive with fetoprotein transcription factor. Nkx2.8 bound to the active AFP promoter, and antisense inhibition of Nkx2.8 mRNA translation selectively reduced expression of both the endogenous human AFP gene and transfected reporters containing the rat AFP promoter.

Molecular Weight: 26 kDa

Gene ID: 26257

NCBI Accession: NP_055175

UniProt: O15522