T-Bet antibody
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- Target See all T-Bet Antibodies
- T-Bet
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This T-Bet antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Purification
- Antibody is purified by peptide affinity chromatography method.
- Immunogen
- Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TBX21.
- Top Product
- Discover our top product T-Bet Primary Antibody
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- Application Notes
- TBX21 antibody can be used for detection of TBX21 by ELISA at 1:312500. TBX21 antibody can be used for detection of TBX21 by western blot at 1.0-2.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
- Concentration
- 1 mg/mL
- Buffer
- Antibody is lyophilized in PBS buffer with 2 % sucrose.
- Handling Advice
- As with any antibody avoid repeat freeze-thaw cycles.
- Storage
- 4 °C/-20 °C
- Storage Comment
- For short periods of storage (days) store at 4 °C. For longer periods of storage, store TBX21 antibody at -20 °C.
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- Target
- T-Bet
- Alternative Name
- TBX21 (T-Bet Products)
- Synonyms
- T-PET antibody, T-bet antibody, TBET antibody, TBLYM antibody, TBT1 antibody, Tbet antibody, Tblym antibody, T-box 21 antibody, TBX21 antibody, Tbx21 antibody
- Background
- TBX21 is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. It is the human ortholog of mouse Tbx21/Tbet gene. Studies in mouse show that Tbx21 protein is a Th1 cell-specific transcription factor that controls the expression of the hallmark Th1 cytokine, interferon-gamma (IFNG). Expression of the human ortholog also correlates with IFNG expression in Th1 and natural killer cells, suggesting a role for this gene in initiating Th1 lineage development from naive Th precursor cells.
- Molecular Weight
- 58 kDa
- Gene ID
- 30009
- NCBI Accession
- NP_037483
- UniProt
- Q9UL17
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