Indian Hedgehog antibody

Catalog No. ABIN2462836

Product Details anti-Indian Hedgehog Antibody

Target: Indian Hedgehog (IHH)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Indian Hedgehog antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human IHH.

Application Details

Application Notes: IHH antibody can be used for detection of IHH by ELISA at 1:312500. IHH antibody can be used for detection of IHH by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store IHH antibody at -20 °C.

Target Details for Indian Hedgehog

Target: Indian Hedgehog (IHH)

Alternative Name: IHH (IHH Products)

Synonyms: BDA1 antibody, HHG2 antibody, X-hh antibody, Xbhh antibody, bhh antibody, bhh-a antibody, IHH antibody, ehh antibody, zgc:113262 antibody, indian hedgehog antibody, Indian hedgehog antibody, indian hedgehog S homeolog antibody, Indian hedgehog homolog b antibody, IHH antibody, Ihh antibody, ihh.S antibody, ihhb antibody

Background: IHH is an intercellular signal essential for a variety of patterning events during development. It binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. It is implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones and induces the expression of parathyroid hormone-related protein (PTHRP).

Molecular Weight: 45 kDa

Gene ID: 3549

NCBI Accession: NP_002172

UniProt: Q14623

Pathways: Hedgehog Signaling