PRIM1 antibody

Catalog No. ABIN2462916

Product Details anti-PRIM1 Antibody

Target: PRIM1 (Primase, DNA, Polypeptide 1 (49kDa) (PRIM1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRIM1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PRIM1.

Application Details

Application Notes: PRIM1 antibody can be used for detection of PRIM1 by ELISA at 1:1562500. PRIM1 antibody can be used for detection of PRIM1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store PRIM1 antibody at -20 °C.

Target Details for PRIM1

Target: PRIM1 (Primase, DNA, Polypeptide 1 (49kDa) (PRIM1))

Alternative Name: PRIM1 (PRIM1 Products)

Synonyms: AI324982 antibody, p49 antibody, dnapol-alpha50 antibody, DNA primase, p49 subunit antibody, DNA primase subunit 1 antibody, DNA primase subunit 1 S homeolog antibody, Prim1 antibody, PRIM1 antibody, prim1.S antibody

Background: The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. PRIM1 is the small, 49 kDa primase subunit.The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the small, 49 kDa primase subunit.

Molecular Weight: 50 kDa

Gene ID: 5557

NCBI Accession: NP_000937

UniProt: P49642

Pathways: Telomere Maintenance, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, SARS-CoV-2 Protein Interactome