NCAPD2
Reactivity: Mouse
WB, IF (cc), IF (p)
Host: Rabbit
Polyclonal
AbBy Fluor® 594
Application Notes
NCAPD2 antibody can be used for detection of NCAPD2 by ELISA at 1:312500. NCAPD2 antibody can be used for detection of NCAPD2 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
Concentration
1 mg/mL
Buffer
Antibody is lyophilized in PBS buffer with 2 % sucrose.
Handling Advice
As with any antibody avoid repeat freeze-thaw cycles.
Storage
4 °C/-20 °C
Storage Comment
For short periods of storage (days) store at 4 °C. For longer periods of storage, store NCAPD2 antibody at -20 °C.
Target
NCAPD2
(Non-SMC Condensin I Complex, Subunit D2 (NCAPD2))
NCAPD2 is the regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. NCAPD2 may target the condensin complex to DNA via its C-terminal domain.