ATG10 antibody

Catalog No. ABIN2463627

Product Details anti-ATG10 Antibody

Target: ATG10 (Autophagy Related 10 (ATG10))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG10 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ATG10.

Application Details

Application Notes: ATG10 antibody can be used for detection of ATG10 by ELISA at 1:312500. ATG10 antibody can be used for detection of ATG10 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store ATG10 antibody at -20 °C.

Target Details for ATG10

Target: ATG10 (Autophagy Related 10 (ATG10))

Alternative Name: ATG10 (ATG10 Products)

Synonyms: APG10 antibody, APG10L antibody, pp12616 antibody, Atg10 antibody, ATG10 antibody, MGC123341 antibody, im:7153729 antibody, zgc:123341 antibody, ATATG10 antibody, autophagy 10 antibody, DDBDRAFT_0190076 antibody, DDBDRAFT_0252608 antibody, DDB_0190076 antibody, DDB_0252608 antibody, 5330424L23Rik antibody, 5430428K15Rik antibody, AI852123 antibody, Agp10 antibody, Apg10l antibody, Apg10p antibody, Atg10l antibody, autophagy related 10 antibody, autophagy-related 10 antibody, AuTophaGy (yeast Atg homolog) antibody, ATG10 autophagy related 10 homolog (S. cerevisiae) antibody, autophagocytosis-associated family protein antibody, E2-like conjugating enzyme antibody, hypothetical protein antibody, autophagy protein 10 antibody, autophagy-related protein 10 antibody, ATG10 antibody, Atg10 antibody, atg-10 antibody, atg10 antibody, CAALFM_C302930WA antibody, APG10 antibody

Background: Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12-ATG5 conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A), a homolog of yeast Apg8, to a membrane-bound form.Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A, MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).

Molecular Weight: 25 kDa

Gene ID: 83734

NCBI Accession: NP_113670

UniProt: Q9H0Y0

Pathways: ER-Nucleus Signaling, Autophagy