SREBF1 antibody

Catalog No. ABIN2463722

Product Details anti-SREBF1 Antibody

Target: SREBF1 (Sterol Regulatory Element Binding Transcription Factor 1 (SREBF1))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SREBF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SREBF1.

Application Details

Application Notes: SREBF1 antibody can be used for detection of SREBF1 by ELISA at 1:62500. SREBF1 antibody can be used for detection of SREBF1 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store SREBF1 antibody at -20 °C.

Target Details for SREBF1

Target: SREBF1 (Sterol Regulatory Element Binding Transcription Factor 1 (SREBF1))

Alternative Name: SREBF1 (SREBF1 Products)

Synonyms: SREBP-1c antibody, SREBP1 antibody, bHLHd1 antibody, ADD-1 antibody, ADD1 antibody, D630008H06 antibody, SREBP-1 antibody, SREBP-1a antibody, SREBP1c antibody, Srebp1 antibody, SCREBP1 antibody, SREBP-1C antibody, SREBF1 antibody, srebp1 antibody, sterol regulatory element binding transcription factor 1 antibody, sterol regulatory element binding transcription factor 1 L homeolog antibody, sterol regulatory element binding protein-1 antibody, Sterol regulatory element Binding Protein antibody, SREBF1 antibody, Srebf1 antibody, srebf1.L antibody, SREBP-1 antibody, srebf1 antibody, sbp-1 antibody

Background: SREBF1 is a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family.This gene encodes a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Two transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 125 kDa

Gene ID: 6720

NCBI Accession: NP_001005291

UniProt: P36956

Pathways: AMPK Signaling, Caspase Cascade in Apoptosis, Negative Regulation of Hormone Secretion, Regulation of Lipid Metabolism by PPARalpha