Fusion protein containing 73 aas from region aa800-900 (according to NP_079511.1). No crossreactivity expected with TANK1.
Purification
TNKS2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
TNKS2 antibody was raised against a 12 amino acid synthetic peptide near the internal of TNKS2.
TNKS2
Reactivity: Human
ELISA
Host: Rabbit
Polyclonal
Biotin
Application Notes
Western Blot: In transfected HEK293 transiently expressing TANK2 a band of approximately 127 kDa is observed. This band is not observed in the non-transfected HEK293. The calculated molecular size is 127 kDa according to NP_079511.1. Recommended concentration, 0.5-1.5 μg/mL. Full image caption: HEK293 overexpressing TANK2 (lane 4) and TANK1 (lane 2) and probed with 42-018 (mock transfection in first lane). Lane three is empty. Lower panel shows the same lysates probed for alpha-Actin to show protein levels. Primary incubation (0.5 μg/mL) was overnight at 4 °C. Detected by chemiluminescence.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.