Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Specificity
This antibody is expected to recognize all three reported isoforms (NP_036472.2, NP_775114.1, NP_001129493.1).
Purification
NFATC2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
NFATC2 antibody was raised against a 15 amino acid synthetic peptide near the internal region of NFATC2.
ELISA: Antibody detection limit dilution 1:64000. Western Blot: Approximately 110 kDa band observed in human Molt4 lysate (calculated MW of 100 kDa according to NP_775114.1). Recommended concentration: 0.3-1 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
NFAT1
(Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 (NFAT1))