COX4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
COX4 antibody was raised against an 11 amino acid synthetic peptide near the C-Terminus of COX4.
COX4I1
Reactivity: Human, Rat
WB, IHC (p), IF (p)
Host: Mouse
Monoclonal
8D8
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:2000. Western Blot: Approximately 17 kDa band observed in human skeletal muscle lysates (calculated MW of 19.6 kDa according to NP_115998.2). Recommended concentration: 1-3 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
COX IV (COX4I1)
(Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1))