Deltex Homolog 3 antibody (Internal Region)

Catalog No. ABIN2464362

Product Details anti-Deltex Homolog 3 Antibody

Target: Deltex Homolog 3 (DTX3) (Deltex Homolog 3 (Drosophila) (DTX3))

Binding Specificity: Internal Region

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Deltex Homolog 3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: DTX3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: DTX3 antibody was raised against a 12 amino acid synthetic peptide near the internal region (near N-Terminus) of DTX3 (near N-Terminus).

Application Details

Application Notes: ELISA: Antibody detection limit dilution 1:8000. Western Blot: Approximately 90 kDa band observed in mouse Fetal brain lysates and in lysates of cell line NIH3T3 (calculated MW of 38.0 kDa according to human NP_848597.1 and 37.98 kDa to mouse NP_109639.1). The observed much higher molecular weight corresponds to earlier findings in literature with different antibodies (Takeyama et al, J Biol Chem. 2003 Jun13,278 (24) :21930-7., PMID: 12670957). Recommended concentration: 0.2-0.5 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C.

Target Details for Deltex Homolog 3

Target: Deltex Homolog 3 (DTX3) (Deltex Homolog 3 (Drosophila) (DTX3))

Alternative Name: DTX3 (DTX3 Products)

Synonyms: RGD1566181 antibody, Deltex3 antibody, mg310 antibody, RNF154 antibody, deltex E3 ubiquitin ligase 3 antibody, deltex homolog 3 (Drosophila) antibody, deltex 3, E3 ubiquitin ligase antibody, Dtx3 antibody, DTX3 antibody, dtx3 antibody

Gene ID: 196403

NCBI Accession: NP_848597

UniProt: Q8N9I9

Pathways: Notch Signaling