GHITM antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
GHITM antibody was raised against a 14 amino acid synthetic peptide near the internal region of GHITM.
GHITM
Reactivity: Human
ELISA
Host: Rabbit
Polyclonal
HRP
Application Notes
ELISA: Antibody detection limit dilution 1:8000. Western Blot: Approximately 35 kDa band observed in HeLa cell lysates (calculated MW of 37.2 kDa according to human NP_055209.2). Recommended concentration: 0.1-0.5 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
GHITM
(Growth Hormone Inducible Transmembrane Protein (GHITM))