This Serotonin Receptor 1A antibody is un-conjugated
Application
Western Blotting (WB), ELISA
Purification
HTR1A antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
HTR1A antibody was raised against a 14 amino acid synthetic peptide near the internal region of HTR1A (aa 249-262).
ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 37 kDa and 35 kDa in human brain (hippocampus, Substantia nigra) lysates after 0.1 μg/mL antibody staining. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.