This antibody is expected to recognize isoform A (NP_000627.2) only. Reported variants represent identical protein: NP_000627.2, NP_001019636.1.
Purification
SOD2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
SOD2 antibody was raised against a amino acid synthetic peptide near the internal region of SOD2 (isoform A).
SOD2
Reactivity: Rat
WB, IHC, ELISA, IP, ICC, IF
Host: Rabbit
Polyclonal
FITC
Application Notes
ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 23 kDa band observed in human brain (cerebellum, cerebral cortex) and HeLa lysates (calculated MW of 24.8 kDa according to NP_000627.2). Recommended concentration: 0.01-0.03 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.