This antibody is expected to recognize both reported isoforms (NP_071878.2, NP_001171275.1). Reported variants represent identical proteins: NP_071878.2, NP_001120823.1
Purification
Sirt3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Sirt3 antibody was raised against a amino acid synthetic peptide near the internal region of Sirt3 (mouse).
SIRT3
Reactivity: Human
WB, IHC, ELISA
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:64000. Western Blot: Approximately 30 kDa band observed in mouse heart lysates (calculated MW of 28.8 kDa according to NP_071878.2). Recommended concentration: 1-3 μg/mL. an additional band of unknown identity was also consistently observed at 15 kDa . This band was successfully blocked by incubation with the immunizing peptide.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.