This antibody is expected to recognize isoform 1 and 2 (NP_005814.2, NP_037536.2).
Purification
MSLN antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
MSLN antibody was raised against a 13 amino acid synthetic peptide near the internal region of MSLN.
MSLN
Reactivity: Human
IHC (f)
Host: Mouse
Monoclonal
Biotin
Application Notes
ELISA: Antibody detection limit dilution 1:64000 Western Blot: Approximately 70 kDa band observed in human placenta lysates (calculated MW of 68.1 kDa according to NP_005814.2 and of 69.0 kDa according to NP_037536.2). Recommended concentration: 0.3-1 μg/mL. an additional band of unknown identity was also consistently observed at 150 kDa . This band was successfully blocked by incubation with the immunizing peptide.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.