This antibody is expected to recognize all three reported isoforms (NP_004815.2, NP_736607.1, NP_736608.1).
Purification
CDYL antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
CDYL antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of CDYL.
CDYL
Reactivity: Human
WB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:4,000. Western Blot: Approximately 75 kDa band observed in human testis lysates (calculated MW of 66.5 kDa according to NP_004815.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Lahn et al, Proc Natl Acad Sci U S A. 2002 Jun 25,99 (13) :8707-12, PMID: 12072557). Recommended concentration: 1-3 μg/mL. an additional band of unknown identity was also consistently observed at 26 kDa . This band was successfully blocked by incubation with the immunizing peptide.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.