CRKL antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
CRKL antibody was raised against a 12 amino acid synthetic peptide near the C-Terminus of CRKL.
CRKL
Reactivity: Human
WB, IF
Host: Mouse
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 37 kDa band observed in K562 and Jurkat lysates (calculated MW of 33.8 kDa according to NP_005198). Recommended concentration: 0.01-0.03 μg/mL. Immunohistochemistry: In paraffin embedded human thymus shows staining of cellular membranes in thymocytes. Recommended concentration, 2.5-3.8 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.