IRF5 antibody (C-Term)

Catalog No. ABIN2465234

Product Details anti-IRF5 Antibody

Target: IRF5 (Interferon Regulatory Factor 5 (IRF5))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This IRF5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)

Specificity: This antibody is expected to recognize both human isoforms.

Purification: IRF5 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: IRF5 antibody was raised against a 12 amino acid synthetic peptide near the C-Terminus of IRF5.

Application Details

Application Notes: ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 57 kDa band observed in A549 lysate . In transfected HEK293 transiently expressing IRF5 a strong band of approximately 60 kDa is observed. This band is not observed in the non-transfected HEK293. The calculated molecular size is 57.8 kDa according to NP_001092099.1. Recommended concentration, 0.1-0.3 μg/mL. IHC: This antibody showed nuclear staining in Human Cervix, as confirmed by anonymous customer (data not shown). Chromatin immunoprecipitation: This antibody has been successfully used in human monocyte-derived dendritic cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C.

Target Details for IRF5

Target: IRF5 (Interferon Regulatory Factor 5 (IRF5))

Alternative Name: IRF5 (IRF5 Products)

Synonyms: IRF5 antibody, irf5 antibody, SLEB10 antibody, AW491843 antibody, mirf5 antibody, im:7155364 antibody, zgc:76986 antibody, interferon regulatory factor 5 antibody, interferon regulatory factor 5 L homeolog antibody, IRF5 antibody, irf5 antibody, Irf5 antibody, irf5.L antibody

Gene ID: 3663

NCBI Accession: NP_002191, NP_116032

UniProt: Q13568

Pathways: TLR Signaling, Autophagy