Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Purification
VMAT2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
VMAT2 antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of VMAT2.
ELISA: Antibody detection limit dilution 1:8,000. Western Blot: A faint band of appr. 50 kDa band observed in human placenta lysates (calculated MW of 55.7 kDa according to NP_003045). Recommended concentration: 0.3-1.0 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
SLC18A2
(Solute Carrier Family 18 (Vesicular Monoamine Transporter), Member 2 (SLC18A2))