Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Specificity
This antibody is expected to recognize both reported isoforms NP_003072 and NP_570824.
Purification
SNAP25 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
SNAP25 antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of SNAP25.
ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 22 kDa band observed in human brain and mouse brain lysates (calculated MW of 23.3 kDa according to NP_003072.2 and NP_570824.1). Recommended concentration: 0.01-0.03 μg/mL. Immunohistochemistry: In paraffin embedded human cerebral cortex shows axon/dendrite staining leaving cell bodies relatively unstained. Recommended concentration, 2-4 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.