TRIM28 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
TRIM28 antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of TRIM28.
TRIM28
Reactivity: Human, Mouse, Rat
IHC, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:8000. Western Blot: Approximately 100-120 kDa band seen in HepG2 lysate. Recommended for use at 0.2-1 μg/mL (Predicted MW Approximately 92 kDa according to NP_005753). Immunohistochemistry: This antibody has been successfully used on human gastric tumor surgical specimens: Yokoe et al, Ann Surg Oncol. 2009 Nov 7. [Epub ahead of print]. (PMID: 19898899). Recommended concentration: 5 μg/mL. Immunofluorescence: Successfully used on mouse embryo at the late zygote stage, 5 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.