This Acetylcholinesterase antibody is un-conjugated
Application
Western Blotting (WB), ELISA
Specificity
This antibody is expected to recognize isoform NP_000656 only (the ubiquitously expressed, hydrophillic form).
Purification
ACHE antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
ACHE antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of ACHE.
AChE
Reactivity: Rat
WB, IHC, ICC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:2000. Western Blot: Approximately 70 kDa band observed in human cerebellum lysates (calculated MW of 67.8 kDa according to NP_000656.1). Recommended concentration: 1-3 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.