ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
Specificity
This antibody is expected to recognize isoforms 1, 2 and 3 (NP_671700.1, NP_005742.4, NP_671714.1 respectively). The isoforms Yotiao, AKAP350B and AKAP350C are not recognized.
Purification
AKAP9 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
AKAP9 antibody was raised against a 12 amino acid synthetic peptide near the C-Terminus of AKAP9.
AKAP9
Reactivity: Human
ELISA
Host: Rabbit
Polyclonal
HRP
Application Notes
ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 450 kDa band observed in HEPG2 lysate (calculated MW of 454 kDa according to NP_ 671700.1). Recommended concentration: 1-3 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
AKAP9
(A Kinase (PRKA) Anchor Protein (Yotiao) 9 (AKAP9))