CCKBR antibody (Internal Region)

Catalog No. ABIN2465511

Product Details anti-CCKBR Antibody

Target: CCKBR (Cholecystokinin B Receptor (CCKBR))

Binding Specificity: Internal Region

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This CCKBR antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Purification: CCKBR antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: CCKBR antibody was raised against a 16 amino acid synthetic peptide near the internal region of CCKBR.

Application Details

Application Notes: ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 75 kDa band observed in purified membranes of rat insulinoma cell line RIN14B (calculated MW of 48.4 kDa according to NP_033957.1). The observed molecular weight also corresponds to earlier findings in literature with different antibodies (Morisset et al J Histochem Cytochem. 2003 Nov,51 (11) :1501-13, PMID: 14566022). Recommended concentration: 0.1-0.3 μg/mL. Immunohistochemistry: In paraffin embedded human pancreas, shows strong staining in islets of Langerhans and weak staining in acinar cells. Recommended concentration, 3 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C.

Target Details for CCKBR

Target: CCKBR (Cholecystokinin B Receptor (CCKBR))

Alternative Name: CCKBR (CCKBR Products)

Synonyms: CCKBR antibody, LOC100232835 antibody, CCK-B antibody, CCK2R antibody, GASR antibody, Cck2r antibody, Cholrec antibody, CCKR-2 antibody, CCK-BR antibody, CCK2-R antibody, CCK-CHR antibody, cholecystokinin B receptor antibody, cholecystokinin receptor-like antibody, CCKBR antibody, LOC100232835 antibody, Cckbr antibody

Gene ID: 887

NCBI Accession: NP_795344

UniProt: P32239

Pathways: Positive Regulation of Peptide Hormone Secretion, Feeding Behaviour