Western Blotting (WB), Immunohistochemistry (IHC), ELISA
Specificity
This antibody also recognizes COX4I2.
Purification
COX4I1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
COX4I1 antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of COX4I1.
COX4I1
Reactivity: Human, Rat
WB, IHC (p), IF (p)
Host: Mouse
Monoclonal
8D8
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:4,000. Western Blot: Approximately 18 kDa band observed in human muscle lysates (calculated MW of 19.6 kDa according to NP_001852.1). Recommended concentration: 0.01-0.03 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
COX IV (COX4I1)
(Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1))