This antibody is expected to recognize EHD1 protein as well as EHD2.
Purification
EHD2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
EHD2 antibody was raised against a 15 amino acid synthetic peptide near the C-Terminus of EHD2.
EHD2
Reactivity: Human, Mouse, Rat
WB, ELISA, IHC, IP
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 60 kDa band observed in human heart lysates (calculated MW of 61.2 kDa according to NP_055416.2). In transfected HEK293 transiently expressing EHD2 a band of approximately 62 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.003-0.01 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.