FHL1 antibody (Internal Region)

Catalog No. ABIN2465696

Product Details anti-FHL1 Antibody

Target: FHL1 (Four and A Half LIM Domains 1 (FHL1))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This FHL1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificity: This antibody is expected to recognise isoforms 2( NP_001440.1) and 5 (NP_001153171.1). Since June 2009 the second N residue in the immunizing peptide is no longer representative as it has been substituted by Q at position 284 of NP_001440.2. Variants NP_001440.2, NP_001153176.1 and NP_001153172.1 represent identical protein.

Purification: FHL1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: FHL1 antibody was raised against a 13 amino acid synthetic peptide near the internal region of FHL1.

Application Details

Application Notes: ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 30 kDa band observed in human muscle lysates (calculated MW of 31.9 kDa according to NP_001440). Recommended concentration: 0.05-0.3 μg/mL. Immunohistochemistry: In paraffin embedded human testis shows fibrous staining in the cytoplasm of developing sperm cells. Recommended concentration, 2-2 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C.

Target Details for FHL1

Target: FHL1 (Four and A Half LIM Domains 1 (FHL1))

Alternative Name: FHL1 (FHL1 Products)

Synonyms: fhl1 antibody, MGC89165 antibody, FHL1 antibody, DKFZp468O1927 antibody, fhl antibody, fhla antibody, zgc:92025 antibody, FHL-1 antibody, FHL1A antibody, FHL1B antibody, FLH1A antibody, KYOT antibody, SLIM antibody, SLIM-1 antibody, SLIM1 antibody, SLIMMER antibody, XMPMA antibody, KyoT antibody, Fhl1 antibody, four and a half LIM domains 1 antibody, four and a half LIM domains 1a antibody, Four and a half LIM domains protein 1 antibody, four and a half LIM domains 1 S homeolog antibody, fhl1 antibody, FHL1 antibody, fhl1a antibody, fhl1.S antibody, Fhl1 antibody

Gene ID: 2273

NCBI Accession: NP_001440

UniProt: Q13642