Fumarase antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Fumarase antibody was raised against a 14 amino acid synthetic peptide near the internal region of Fumarase.
ELISA: Antibody detection limit dilution 1:64,000. Approximately 48 kDa band observed in human kidney, liver and lung lysates (calculated MW of 54.6 kDa according to NP_000134.2). The observed molecular weight corresponds to earlier findings with antibodies from different sources. In transfected HEK293 transiently expressing Fumarase a band of approximately 55 kDa is observed. This band is not observed in the non-transfected HEK293.Recommended concentration: 0.01-0.03 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.