GRIK1 antibody (Internal Region)

Catalog No. ABIN2465767

Product Details anti-GRIK1 Antibody

Target: GRIK1 (Glutamate Receptor, Ionotropic, Kainate 1 (GRIK1))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This GRIK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: This antibody is expected to recognize both reported isoforms according to NP_000821.1 and NP_783300.1.

Purification: GLuR5 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: GLuR5 antibody was raised against a 12 amino acid synthetic peptide near the internal region (near the C-Terminus) of GLuR5.

Application Details

Application Notes: ELISA: Antibody detection limit dilution 1:8,000. Western Blot: Approximately 150 kDa band observed in human brain (cerebellum and cerebral cortex) lysates (calculated MW of 104 kDa according to NP_000821.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Christensen et al, J Neurosci. 2004 Oct 13,24 (41) :8986-93., PMID: 15483117). Recommended concentration: 1-3 μg/mL. an additional very strong band of unknown identity was also consistently observed at 60 kDa . This band was successfully blocked by incubation with the immunizing peptide.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C.

Target Details for GRIK1

Target: GRIK1 (Glutamate Receptor, Ionotropic, Kainate 1 (GRIK1))

Alternative Name: GLuR5 (GRIK1 Products)

Synonyms: GRIK1 antibody, grik1 antibody, fv36b03 antibody, wu:fv36b03 antibody, EAA3 antibody, EEA3 antibody, GLR5 antibody, GLUR5 antibody, GluK1 antibody, A830007B11Rik antibody, D16Ium24 antibody, D16Ium24e antibody, GluK5 antibody, Glur-5 antibody, Glur5 antibody, Glurbeta1 antibody, GluR5 antibody, glutamate ionotropic receptor kainate type subunit 1 antibody, glutamate receptor, ionotropic, kainate 1a antibody, glutamate receptor, ionotropic, kainate 1 antibody, GRIK1 antibody, grik1a antibody, Grik1 antibody

Gene ID: 2897

NCBI Accession: NP_000821, NP_783300

UniProt: P39086

Pathways: Synaptic Membrane, Regulation of long-term Neuronal Synaptic Plasticity