This antibody recognizes the longer isoform of this protein in A431 lysate.
Purification
GRB7 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
GRB7 antibody was raised against a 14 amino acid synthetic peptide near the N-Terminus of GRB7.
Verified
GRB7
Reactivity: Human
WB, ELISA
Host: Goat
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 60 kDa band observed in lysates of cell line A431 (calculated MW of 59.7 kDa according to NP_005301.2). In transfected HEK293 transiently expressing GRB7 a band of approximately 60 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
GRB7
(Growth Factor Receptor-Bound Protein 7 (GRB7))