This antibody is expected to recognize both reported isoforms (NP_783195.2, NP_006522.2)
Purification
IFT88 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
IFT88 antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of IFT88.
IFT88
Reactivity: Human
WB, IF, ICC
Host: Rabbit
Polyclonal
PerCP
Application Notes
ELISA: Antibody detection limit dilution 1:32000. Western Blot: Preliminary experiments in human kidney, liver and lung lysates gave no specific signal but low background (at antibody concentration up to 1 μg/mL). Immunohistochemistry: In paraffin embedded human kidney shows staining of the brush border in PCT. Recommended concentration, 3 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
IFT88
(Intraflagellar Transport 88 Homolog (IFT88))