Western Blotting (WB), Immunohistochemistry (IHC), ELISA
Purification
NONO antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
NONO antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of NONO.
NONO
Reactivity: Human, Mouse, Rat, Dog, Horse, Cow, Guinea Pig
WB, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 60 kDa band observed in mouse brain lysates (calculated MW of 54.2 kDa according to NP_031389.3). Recommended concentration: 0.1-0.3 μg/mL. Immunohistochemistry: In paraffin embedded human spleen shows nuclear staining in select splenocytes. Recommended concentration, 3-6 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.