PDK1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
PDK1 antibody was raised against an 11 amino acid synthetic peptide near the internal region of PDK1.
PDPK1
Reactivity: Human
WB, ELISA
Host: Mouse
Monoclonal
2E2
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:1,000. Western Blot: Approximately 65-70 kDa band observed in rat heart lysates (calculated MW of 49.2 kDa according to NP_002601.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 1-3 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
PDPK1
(3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))