PRPF31 antibody (Internal Region)

Catalog No. ABIN2466149

Product Details anti-PRPF31 Antibody

Target: PRPF31 (Pre-mRNA Processing Factor 31 (PRPF31))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PRPF31 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: PRPF31 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: PRPF31 antibody was raised against a 15 amino acid synthetic peptide near the internal region of PRPF31.

Application Details

Application Notes: ELISA: Antibody detection limit dilution 1:128,000. Western Blot: Approximately 60 kDa band observed in lysates of cell lines A431 and HeLa (calculated MW of 55.4 kDa according to NP_056444.2). Recommended concentration: 1-3 μg/mL. an additional band of unknown identity was also consistently observed at 35 kDa . This band was successfully blocked by incubation with the immunizing peptide. Also a band of 28 kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western Blot.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C.

Target Details for PRPF31

Target: PRPF31 (Pre-mRNA Processing Factor 31 (PRPF31))

Alternative Name: PRPF31 (PRPF31 Products)

Synonyms: DDBDRAFT_0219465 antibody, DDBDRAFT_0304959 antibody, DDB_0219465 antibody, DDB_0304959 antibody, PRPF31 antibody, NY-BR-99 antibody, PRP31 antibody, RP11 antibody, SNRNP61 antibody, prp31 antibody, rp11 antibody, 1500019O16Rik antibody, 2810404O06Rik antibody, AW554706 antibody, zgc:66177 antibody, pre-mRNA processing factor 31 antibody, PrpF31 U4/U6*U5 snRNP-associated pre-mRNA processing factor 31, (IC) antibody, U4/U6 small nuclear ribonucleoprotein Prp31 antibody, pre-mRNA processing factor 31 S homeolog antibody, PRP31 pre-mRNA processing factor 31 homolog (yeast) antibody, PRPF31 antibody, prpf31 antibody, PrpF31 antibody, LOC100455616 antibody, Prpf31 antibody, prpf31.S antibody

Gene ID: 26121

NCBI Accession: NP_056444

UniProt: Q8WWY3

Pathways: Ribonucleoprotein Complex Subunit Organization