This antibody is expected to recognize both transcript variants of this protein (as represented by NP_054730 and NP_659198) which encode the same protein.
Purification
SOCS5 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
SOCS5 antibody was raised against an 11 amino acid synthetic peptide near the N-Terminus of SOCS5.
SOCS5
Reactivity: Human
WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro), ICC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Experiments gave bands at approximately 48 kDa and 24 kDa in human brain lysate after 0.3 μg/mL antibody staining.2 kDa according to NP_054730 and NP_659198. Both detected bands were successfully blocked by incubation with the immunizing peptide. Approximately 65 kDa band observed in Molt4 lysate (calculated MW of 61.2 kDa according to NP_054730.1). Recommended concentration: 0.3-1 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
SOCS5
(Suppressor of Cytokine Signaling 5 (SOCS5))