ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
Specificity
This antibody is expected to recognize isoform 1 (NP_000359.1) only.
Purification
TSC1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
TSC1 antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of TSC1.
TSC1
Reactivity: Mouse
DB
Host: Rabbit
Polyclonal
RB41773
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:128000. Western Blot: Approximately 150 kDa band observed in human brain lysates (calculated MW of 130 kDa according to NP_000359.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Miloloza et al Hum Mol Genet. 2000 Jul 22,9 (12) :1721-7, PMID: 10915759). In transfected HEK293 transiently expressing human TSC1 a band of approximately 120 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-0.3 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.