Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Purification
VPS25 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
VPS25 antibody was raised against a 10 amino acid synthetic peptide near the internal region of VPS25.
VPS25
Reactivity: Human
ELISA
Host: Rabbit
Polyclonal
Biotin
Application Notes
ELISA: Antibody detection limit dilution 1:1,000. Western Blot: Approximately 20-22 kDa kDa band observed in human duodenum, kidney and liver lysates (calculated MW of 20.7 kDa according to NP_115729.1). Recommended concentration: 0.5-1.5 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
VPS25
(Vacuolar Protein-Sorting-Associated Protein 25 (VPS25))