BHMT antibody (C-Term)

Catalog No. ABIN2466485

Product Details anti-BHMT Antibody

Target: BHMT (Betaine--Homocysteine S-Methyltransferase (BHMT))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This BHMT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purification: BHMT antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: BHMT antibody was raised against a 12 amino acid synthetic peptide near the C-Terminus of BHMT.

Application Details

Application Notes: ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 45 kDa band observed in human liver, mouse liver and rat liver lysates (calculated MW of 44.9 kDa according to human NP_001704.1, 45.0 kDa according to mouse NP_057877.1 and rat NP_110477.1). In transfected HEK293 transiently expressing BHMT a band of approximately 49 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.03-0.1 μg/mL. Immunohistochemistry: In paraffin embedded human kidney shows strong cytoplasm staining in PCT cells. Recommended concentration, 2-4 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C.

Target Details for BHMT

Target: BHMT (Betaine--Homocysteine S-Methyltransferase (BHMT))

Alternative Name: BHMT (BHMT Products)

Synonyms: BHMT1 antibody, hm:zehn2153 antibody, wu:fb53h01 antibody, wu:fb63c08 antibody, wu:fj64d01 antibody, zgc:123027 antibody, betaine--homocysteine S-methyltransferase antibody, betaine--homocysteine S-methyltransferase L homeolog antibody, betaine-homocysteine methyltransferase antibody, betaine-homocysteine S-methyltransferase antibody, BHMT antibody, bhmt.L antibody, Bhmt antibody, bhmt antibody

Gene ID: 635

NCBI Accession: NP_001704

UniProt: Q93088

Pathways: Methionine Biosynthetic Process