Apolipoprotein B antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Apolipoprotein B antibody was raised against a 12 amino acid synthetic peptide near the internal region of Apolipoprotein B.
APOB
Reactivity: Human
WB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Bands corresponding to the exopected sizes of ApoB100 and ApoB48 were detected in human serum samples from five different individuals. Recommended concentration: 0.3-1 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.